SubtiBank SubtiBank
yhfW [2020-04-16 17:12:13]
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yhfW [2020-04-16 17:12:13]

similar to Rieske 2Fe-2S iron-sulfur protein
locus
BSU_10390
pI
5.66
mw
56.94 kDa
protein length
509 aa Sequence Blast
gene length
1530 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.1|Electron transport and ATP synthesis] → [category|SW 2.1.4|Electron transport/ other/ based on similarity]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.7|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.3|Genetic competence]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

    • Gene

    Coordinates
    1,114,057 1,115,586

    Phenotypes of a mutant

  • reduced transfomation efficiency [pubmed|32249531]
  • reduced amounts of citric acid cycle intermediates [pubmed|32249531]

    • The protein

    [SW|Domains]

  • Rieske domain (aa 423-509) (according to UniProt)

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]

    • Structure

  • [PDB|4P9S] (dimethylglycine dehydrogenase from Rattus norvegicus, corresponds to aa 22 ... 396, 25% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|96136B1EDFAA1A34ED012CB5BA1DDFB8A95FB4A1|yhfW]
    description
    [Pubmed|15033535]

    sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]

    • regulation

  • expressed during sporulation in the forespore ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) [Pubmed|15699190]
  • highly upregulated in competent cells as compared to non-competent cells [pubmed|32249531]
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    Biological materials

    Mutant

  • MGNA-A716 (yhfW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/716 NBRP B. subtilis, Japan]
  • BKE10390 ([gene|96136B1EDFAA1A34ED012CB5BA1DDFB8A95FB4A1|yhfW]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE10390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT, downstream forward: _UP4_TAAACCGTTCATTTTAATGA
  • BKK10390 ([gene|96136B1EDFAA1A34ED012CB5BA1DDFB8A95FB4A1|yhfW]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK10390 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGCCGTTATACCTCCAAT, downstream forward: _UP4_TAAACCGTTCATTTTAATGA

    • References

  • 16497325,15033535,15699190,32249531